By Mike Esterl And Tripp Mickle 

PepsiCo Inc. said Friday it would remove the sweetener aspartame from Diet Pepsi, to address consumer concerns about the artificial additive and reverse slumping diet soda sales.

PepsiCo said it would switch to sucralose, a less controversial but still artificial sweetener. The beverage giant said the change--the boldest soda reformulation since Coca-Cola Co.'s New Coke fiasco--was in response to consumer surveys showing aspartame as the No. 1 reason Americans are shunning diet colas.

Diet Pepsi volumes fell 5.2% in the U.S. last year, according to industry tracker Beverage Digest, while U.S. volumes of the market's leading diet cola, Diet Coke, plunged 6.6% last year.

In addition to trying to stem the free-fall, Diet Pepsi is taking direct aim at rival brand Diet Coke. In announcing the change, PepsiCo repeatedly described the new Diet Pepsi as "aspartame-free." In a statement, Seth Kaufman, a senior vice president of PepsiCo North America Beverages, said: "Diet cola drinkers in the U.S. told us they wanted aspartame-free Diet Pepsi and we're delivering."

A payoff from the substitution is hardly guaranteed. Not only is it likely to change the taste of Diet Pepsi, it also comes at a time when consumers are showing preferences for natural foods. Pepsi is swapping one artificial sweetener (sucralose, better known as Splenda) for another (aspartame, known as Equal and Nutrasweet). That said, sucralose hasn't come under as much consumer fire as aspartame because of worries about health effects.

The new sweetener is a blend of sucralose and acesulfame potassium that will be used in Diet Pepsi, Caffeine Free Diet Pepsi and Wild Cherry Diet Pepsi in the U.S. beginning in August. The new sweetener formulation "was developed after extensive research and testing with U.S. diet cola drinkers," the company said.

Until a few years ago, diet soda was the industry's great hope as weight-conscious Americans dialed back on full-calorie soft drinks. But longtime drinkers have been switching to bottled water. Last year, U.S. diet soda volumes fell 5.9% while full-calorie soda volumes rose 0.9%, according to Beverage Digest.

Diet Pepsi has the added challenge of being runner-up. It had a U.S. soda market share of 4.3% last year, barely half of Diet Coke's 8.5%.

"The big question is does Coke follow, because that's the big brand, but they would be following in Pepsi's footsteps. There's tension involved, " said Dean Crutchfield, an independent branding consultant.

The move appeared to catch Coca-Cola by surprise. Coke Chief Executive Muhtar Kent was appearing on CNBC when the news was announced Friday morning. When asked about it, he didn't say whether the company would also consider removing aspartame from Diet Coke.

"I'm not here to defend aspartame," he said during the interview, but then cited a recent report by the European Food Safety Authority saying aspartame is one of the most "researched ingredients in the world and safe."

Later Friday, Coca-Cola issued a statement saying it didn't have any plans to change the sweetener in Diet Coke.

Changing formulas is risky business as Coke found when it announced it was changing its flagship brand in 1985. In an epic marketing failure, diehard Coke consumers rebelled against the new Coke and the company had to bring back the old one.

The Center for Science in the Public Interest, a public health group and soft drink critic, said Friday that, while consumers should still avoid acesulfame potassium, more commonly known as Ace K, sucralose is "likely" a safer sweetener than aspartame. It cited past studies suggesting aspartame had caused tumors in rats, and said tests in the 1970s flagged Ace K as a potential cancer risk.

PepsiCo said it continues to stand behind the safety of aspartame and will keep using it outside the U.S. and in other diet offerings, including Diet Mountain Dew and Pepsi Max, a diet cola marketed more aggressively to males.

Aspartame "doesn't even hit top 10" among concerns of Pepsi Max drinkers, Mr. Kaufman said in an interview. He said aspartame "remains an important sweetener option for us."

Aspartame, developed by a G.D. Searle & Company researcher in 1965, has been under suspicion almost from its inception. But federal authorities in the U.S. and Europe have repeatedly found aspartame to be safe.

The Food and Drug Administration in 2007 discredited a study by the European Ramazzini Foundation of Italy that labeled aspartame as a carcinogen. The European Food and Safety Association in 2013 completed a full review of aspartame and ruled out any associated risk of genetic damage or cancer. It also determined it doesn't harm the brain or developing infants during pregnancy.

The American Heart Association and American Diabetes Association said in 2012 that artificial sweeteners, including aspartame, "when used judiciously...could facilitate reductions in added sugar" and help with weight loss.

But criticism--particularly in social media--flared up last year due in part to a study in the journal Nature. The study, published in September, indicated zero-calorie sweeteners such as saccharin, sucralose and aspartame can change bacteria in the gut and trigger higher blood glucose levels, a risk factor for diabetes.

Major aspartame makers include Japan's Ajinomoto Co., Inc., Korea's Daesang Corp. and a host of Chinese companies such as SinoSweet Co., Ltd. and VitaSweet Co. Ltd.

The NutraSweet Company, which is based in Augusta, Ga., made the sweetener a popular brand in the U.S. The company, owned by Boston-based private-equity firm J.W. Childs Associates LP, last year announced it would stop making aspartame and close its Augusta plant. The plant currently is idle, NutraSweet chief executive William DeFer said on Friday.

Volumes for artificial sweeteners, an estimated $2 billion market, have been declining in the U.S. and Western Europe since about 2008. From that time, aspartame, the third-largest artificial sweetener in the world by volume has seen its volumes decline 2% annually in North America, according to IHS Chemical, an analytics firm.

IHS Chemical Director of Specialty Chemicals Research Marifaith Hackett said that much of that volume decline is attributable to declines in diet soda consumption.

The top two sweeteners are sodium cyclamate, popular in China but banned in the U.S.. and saccharin, known as Sweet 'N Low.

Sucralose, also one of the largest sweeteners by volume and best known as Splenda, is made by U.K.-based Tate & Lyle PLC. The company last year reported a 5% increase in sweetener volumes. Its competitors include Chinese companies like Niutang Chemical Plant Co. Ltd. and JK Sucralose Inc.

This week, Tate & Lyle announced it planned to restructure its sucralose division by shuttering a Singapore plant and consolidating production into a McIntosh, Ala., plant. It said it aims to make Splenda a "low-cost and sustainable business."

Coke and Pepsi have both been trying to solve the diet sweetener problem for years. Pepsi tweaked Diet Pepsi a couple of years ago to a blend that retained aspartame but mixed it with acesulfame potassium.

They have both spent heavily in recent years trying to develop zero-calorie sweeteners that can be marketed as natural, not artificial. Each has placed bets on stevia, which is derived from a plant but can leave a bitter aftertaste.

Coke and Pepsi rolled out cola variations sweetened with stevia in the U.S. last year, but mixed in sugar, turning them into mid-calorie colas instead of diet colas.

Write to Mike Esterl at mike.esterl@wsj.com and Tripp Mickle at Tripp.Mickle@wsj.com

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