SAO PAULO--Brazilian tycoon Eike Batista has signed a definitive agreement to sell a majority stake in another of his key assets as he advances a plan to shore up his heavily-indebted companies.

Mining company MMX Mineracao e Metalicos S.A. (MMXM3.BR), which is controlled by Mr. Batista, said Monday that Abu Dhabi's Mubadala Development Co., already a big investor in Mr. Batista's industrial group, and Dutch investment fund Trafigura Group have agreed to pay $400 million to buy a majority stake in a port in Itaguai, in the south of Rio de Janeiro state.

It is the latest sale by Mr. Batista, who has seen his personal wealth collapse as a result of the financial and operational troubles faced by his group of companies. One of the richest men in the world just over a year ago, with an estimated fortune of more than $30 billion, he is now off the billionaires list altogether.

Trafigura and Mubadala have agreed to buy 65% of MMX Porto Sudeste Ltda, and MMX will retain the remaining 35% stake. The port company will also take on 1.3 billion Brazilian reais in debts owed by one of MMX's mining subsidiaries.

Construction of the port, known as Superporto Sudeste, began in July 2010 and it is expected to begin operations in mid-2014, with an initial capacity to handle 50 million tons of iron ore per year.

The latest sale is evidence that some parts of Mr. Batista's floundering empire still have some value. The group began to unravel in mid-2012, after the flagship oil company, OGX Petroleo e Gas Participacoes SA, began to show signs that it wouldn't be able to live up to promises on oil production.

OGX has since said that it has halted development of most of its oil fields. The firm is negotiating the restructuring of some $3.6 billion in bonds, having defaulted on a $45 million payment at the beginning of October.

OGX's troubles shattered much of the confidence across much of Mr. Batista's interlocking industrial group. Many of the companies are capital-intensive start-ups that have yet to turn a profit.

Yet the former billionaire has been able to find some buyers. On Monday, EIG Management Co. took control of another logistics company, LLX Logistica SA, which is building a different port in the north of Rio de Janeiro state, in exchange for an investment of up to $560 million. In July, Mr. Batista sold a significant stake in his electric-power-generation company MPX Energia SA to German utility E.ON, although he continues to share control.

Mubadala itself has already invested around $1.5 billion in Mr. Batista's holding company EBX.

Write to Matthew Cowley at matthew.cowley@wsj.com

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