Bruker’s Novel maXis ETD Sets Dramatic New Records for Intact Protein Characterization and Top-down Sequencing at ASMS
23 May 2010 - 9:00PM
Business Wire
At the 58th ASMS Conference, Bruker and several collaborating
scientists will present a number of milestone achievements in the
analysis of intact proteins, including the direct sequencing and
characterization using Bruker’s new maXis ETD system. The
high mass accuracy, resolving power and dynamic range which are
essential to dissect complex patterns of product ions in protein
fragmentation allowed, for the first time, performing ETD-based
tandem mass spectrometry on biomolecular ions above 60 kDa
routinely. These results represent a new record for the mass
spectrometric sequencing of intact proteins and unequivocally
demonstrate the dramatic advantages of maXis ETD technology
in ETD-based top-down protein structural analysis.
“An increasing proportion of new therapies in pharmaceutical
development are proteins. At the same time, many first-generation
biotherapeutics will soon come off patent protection, and thus the
need for rapid and reliable total protein characterization has
never been higher,” commented Dr. Michael Schubert, Executive Vice
President at Bruker Daltonics. “We believe that the new maXis
ETD has unique and unprecedented capabilities that far exceed
what’s feasible with intact protein ETD on ion trap/orbitrap hybrid
instruments.”
Since the launch of the novel ultra-high resolution (UHR) QqTOF
instrument maXis™ at ASMS 2008, a number of leading
scientists have taken the opportunity to explore the potential of
the unique combination of high mass range, resolution and speed in
the direct LCMS examination of recombinant proteins. “The results
are extraordinary, and maXis stands alone in this type of
analysis,” commented Bruker’s Head of LCMS Applications Development
Dr. Carsten Baessmann. “Isotopic definition of proteins over 30kDa
and at part-per million mass accuracy under LC conditions are
straight-forward now on our maXis ETD, making the
confirmation of correct expression unequivocal.”
Several examples will be demonstrated at Bruker’s Users Meeting
on Sunday and during the ASMS scientific program. The recent
transfer of Bruker’s leading ETD technology to maXis has
allowed precise intact protein analysis as well as comprehensive
direct protein gas-phase sequencing. An example of particular note
is the direct ETD sequence analysis of intact proteins like
serotransferrin and monoclonal antibodies up to a MW of 150 kDa
featured by Professor Yury Tsybin of the EPFL (Swiss Federal
Institute of Technology Lausanne) in his Thursday 8:30 AM oral
presentation at ASMS. Further examples of top-down intact protein
identification and characterization will be presented in various
poster sessions at ASMS.
The maXis ETD will be highlighted at Bruker's Users
Meeting on Sunday, May 23rd, 2010 before the 58th ASMS Conference
on Mass Spectrometry in Salt Lake City, Utah (USA). For more
information on Bruker’s User Meeting and other ASMS activities,
please visit www.bdal.com/asms2010.
For More Information:
For more information on maXis ETD please visit
www.bdal.com/maxis
For information about Bruker Daltonics and Bruker Corporation
(NASDAQ: BRKR), please visit www.bruker.com
Bruker (NASDAQ:BRKR)
Historical Stock Chart
From Jun 2024 to Jul 2024
Bruker (NASDAQ:BRKR)
Historical Stock Chart
From Jul 2023 to Jul 2024