Washington University Researchers Select Bruker 12 Tesla FTMS and maXis™ UHR-TOF Technologies for Top-Down Proteomics
08 January 2009 - 11:00PM
Business Wire
Bruker Daltonics announced today that the laboratory of Professor
Michael Gross at Washington University in St. Louis has ordered a
package consisting of a Bruker 12 Tesla FTMS and a maXis UHR-TOF,
primarily for applications in top-down proteomics in close
collaboration with Professor Reid Townsend, who heads proteomics
efforts at Washington University. The researchers also plan to use
the maXis for other important applications including accurate mass
formula determination of small molecules and protein structure
studies that employ their unique methods called PLIMSTEX, SIMSTEX,
and FPOP (OH radical footprinting). In top-down proteomics, intact
proteins are first measured to determine their molecular weights,
then selected proteins are fragmented in the gas phase, and the
fragments are again measured with the ultra-high resolution and
mass accuracy of FTMS. The latest Bruker ESI-Qq-FTMS with a high
field 12 Tesla magnet features a mass resolving power of two
million (2,000,000 FWHM) and is a superb tool for analyzing very
complex spectra in top-down proteomics. By correlating the expected
weights of the fragments from the protein sequence with the
experimental results, the location of important protein
post-translational modifications (PTMs) such as phosphorylation or
glycosylation can be unambiguously determined. This functionally
important information about protein modifications is often lost in
more traditional bottom-up proteomics. The Bruker FTMS provides not
only the highest mass resolving power and mass accuracy available
on any mass spectrometer for such measurements, but also offers the
most versatile suite of tools for fragmenting the proteins. Besides
the novel Continuous Accumulation of Selected Ions (CASI�) which
allows researchers to enrich selectively the signal from a selected
protein or one of its large multiply charged fragments, the Bruker
FTMS already includes external collision-cell or internal ICR cell
Collision Induced Dissociation (CID), as well as Electron Capture
Dissociation (ECD) in the ICR cell. Critical to the top-down
analysis process are also software tools that eliminate tedious
manual data interpretation, such as the unique Bruker SNAP �
algorithm. Furthermore, an agreement between Bruker and the
laboratory of Prof. Gross gives his lab early access to the unique,
newly developed front-end Electron Transfer Dissociation (ETD)
module for FTMS, a new, sensitive and easy-to-use method for
fragmenting peptides and proteins in a fast non-ergodic process
that again preserves PTMs. Bruker is the world-leader in ETD on its
HCT high-capacity ion traps, and has now made this sensitive ETD
capability available also on its ultra-high resolution ESI-FTMS
systems as a work-in-progress research capability. Dr. Michael
Gross, a professor in the departments of chemistry, immunology and
medicine, and the Editor of the Journal of the American Society for
Mass Spectrometry, commented: �We and our collaborators at the
Washington University Medical School have many scientific questions
that can best be addressed by top-down proteomics. The number and
nature of protein modifications are critically important in
understanding protein function. We are excited to be working with
Bruker Daltonics on their high-field FTMS for top-down proteomics,
using the unique combination of existing CID and in-cell ECD tools,
as well as on the further development and applications of the new
front-end ETD capability. We expect the acquisition of this
instrument to help us solve biologically and medically important
problems using the top-down approach, and it will put us in a
leadership position in the applications of top-down proteomics.�
The new Bruker ultra-high resolution time-of-flight (UHR-TOF) mass
spectrometer maXis is the first and only instrument available today
that enables the previously incompatible trends in modern mass
spectrometry of ultra-high mass resolution and very fast UHPLC
chromatographic separations. Exceptional mass accuracy of better
than 1 ppm in MS and MS/MS mode, ultra-high mass resolving power of
40,000 to 60,000 at up to 20 Hz, dynamic range over 5 orders of
magnitude, high m/z capabilities, exceptional stability allowing
1.5 mDa high-resolution extracted ion chromatograms, as well as
outstanding sensitivity are all maintained simultaneously even when
coupled to the fastest available chromatography separation systems,
delivering a powerful mass spectrometer for a broad range of
life-science applications. maXis has already been proven as an
exceptional tool in quantitative proteomics, peptide and intact
protein analysis, but also for rapid small molecule identification
using the unique SmartFormula� 3D method for on-the-fly unambiguous
molecular formula read-out, co-developed with Pfizer, UK. �We are
delighted that Professor Gross, who has an outstanding record in
the development of mass spectrometry for new applications, has
chosen Bruker Daltonics as a partner in developing applications in
top-down proteomics,� commented Dr. Gary Kruppa, Bruker Daltonics�
Vice President for Business Development. �We view our ESI-FTMS with
high field magnets as the ultimate platform for top-down proteomics
and are pleased to collaborate with Professor Gross in this field.
The maXis as a high-throughput, high-performance research tool for
applications in proteomics and small molecule research, and the
collaboration on the development of front-end ETD, make this a very
exciting and important research collaboration for Bruker.�
Professor Gross observed further: �The maXis will have many
applications in our laboratory. The ultra-high resolution of this
instrument will allow us to do experiments we have developed in our
lab such as PLIMSTEX, SIMSTEX, and other protein mapping strategies
at high speed with isotopic resolution of proteins up to 40 kDa. We
are very excited about applying the maXis to this work, but we also
see that the maXis will be a superb instrument for the more routine
work done in our facility, such as confirmation of small synthetic
molecules important in chemistry and metabolomics by using accurate
mass MS and MS/MS. We are pleased about bringing new tools such as
SmartFormula� 3D to our facility and making them available to our
collaborators and clients.� ABOUT BRUKER DALTONICS For more
information about Bruker Daltonics and Bruker Corporation (NASDAQ:
BRKR), please visit www.bdal.com and www.bruker.com.
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