PreOmics Introduces a Novel BeatBox™ FFPE Workflow for Accelerated, In-depth Tissue Proteomics
14 April 2023 - 9:00PM
Business Wire
- Introduction of a FFPE workflow extends the tissue proteomics
applications of PreOmics’ unique BeatBox™ technology
- Launch of novel FFPE workflow enables high-throughput, robust
and fast processing of tissue samples
- Bruker and PreOmics to co-commercialize BeatBox workflows
At the AACR Annual Meeting 2023, PreOmics GmbH announced the
launch of a BeatBox-based FFPE workflow, which simplifies, speeds
up and standardizes FFPE sample preparation for deep, unbiased
tissue proteomics by subsequent protein digestion and nanoLC-MS/MS
analysis. The novel BeatBox workflow eliminates the need for
xylene-based deparaffinization and enables fast and robust
processing of up to 96 samples in parallel for discovery research,
as well as for reproducible larger cohort translational and
preclinical research and validation.
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BeatBox is a dedicated system for
convenient, semi-automated sample homogenization of many tissue
types and cells (Photo: Business Wire)
The BeatBox™ tissue homogenizer and cell lyser is a compact,
affordable and stand-alone benchtop system for convenient,
semi-automated sample homogenization of various tissue types and
cells. With flexible input amounts from 1-50 mg, it consistently
delivers reproducible results for LC-MS-based proteomics with
scalable throughput from 1-96 samples. Already successful for fresh
tissue and cell-line proteomics, with the novel FFPE protocol the
BeatBox can now efficiently process FFPE samples for downstream
tissue proteomics. Compared to sonication devices, the BeatBox
requires no complex handling or additional water conditioning. FFPE
or fresh frozen tissue homogenization takes just 10 minutes with
push-button operation.
In a benchmark study, FFPE as well as fresh frozen mouse cardiac
muscle, kidney, and liver tissue (provided by the Research
Institute of Molecular Pathology in Vienna and the Histology
Facility at Vienna BioCenter Core Facilities) was processed using
the novel BeatBox FFPE workflow, as well as with a sonication
approach. Peptides were analyzed on a timsTOF-HT mass spectrometer
(Bruker Daltonics) in DIA-PASEF mode using a 30-minute nLC
gradient. The BeatBox-based FFPE workflow was shown to increase the
proteomic depth for FFPE, as well as for fresh frozen tissue on
average by 14% to 43%, depending on tissue type. Typical
Coefficients of Variation (CV) within replicates were below 10%,
emphasizing the high repeatability of the homogenization process
with the compact BeatBox instrument. Furthermore, the novel
approach saves up to 4 hours of time in comparison to the
sonication workflow, enabling parallelized high-throughput
processing of 96 samples within one working day.
Dr. Michael Wierer, Director of the Proteomics Research
Infrastructure at the University of Copenhagen, states: “We
routinely analyze a large number of tissue samples at once, and to
improve our efficiency, we have successfully adapted the BeatBox
technology for tissue homogenization. Our latest discovery is that
the BeatBox technology can efficiently homogenize paraffin-embedded
tissue scrolls. This breakthrough now allows us to process large
cohorts of FFPE tissue quickly, reproducibly, and with greater
precision, opening up the possibility of large-scale biobank
projects. By leveraging this technology, we are excited to uncover
novel deep proteomics insights in the field of pathology
research.”
Dr. Marcello Stein, Chief Marketing Officer of PreOmics,
commented: “With our novel BeatBox-based FFPE workflow, researchers
finally can simplify, speed-up and standardize FFPE sample
preparation without the need of xylene-based deparaffinization to
fully exploit its potential for unbiased, deep proteomics. We
believe this is a leap forward for the preparation and subsequent
proteomic analysis of large sample cohorts.”
Bruker Corporation (Nasdaq: BRKR) and PreOmics have a commercial
agreement, enabling Bruker to offer the BeatBox-based tissue
workflows for sale with Bruker's timsTOF Pro mass spectrometer.
Have a look yourself: PreOmics representatives will be at the
AACR Bruker booth #1818 to showcase the BeatBox and FFPE
application note. The application note can be accessed at
https://www.preomics.com/applications/ffpe.
About PreOmics GmbH
PreOmics empowers our clients in life sciences to establish
biological knowledge through efficient, reliable solutions and
workflows that set the standard for protein analysis. Our team
spirit, energy, and commitment empower us to be both creative and
quality focused – A trusted partner with deeply rooted scientific
experience. We envisage a future with revolutionary proteomic
discovery processes open for everyone. Tools that reveal hidden
causes of diseases, ensure sustainable nutrition, and provide
diagnoses that enhance lives and society.
Website: www.preomics.com Address: Am Klopferspitz 19 | 82152
Planegg/Martinsried | Germany
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version on businesswire.com: https://www.businesswire.com/news/home/20230414005064/en/
Dr. Garwin Pichler Founder & Managing Director PreOmics GmbH
Email: info@preomics.com
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