By Tom Fairless and Ruth Bender 

BRUSSELS--European Union regulators have suspended their review of Orange SA's EUR3.4 billion ($3.62 billion) acquisition of Spanish broadband and cellphone operator Jazztel SA for a second time as they seek more information to help decide the case.

The move injects fresh uncertainty into a deal that would almost double Orange's market share in broadband Internet access in Spain, leapfrogging Vodafone Group PLC to become the country's second-largest provider in that segment. Both companies trail the market leader, Telefónica SA.

A spokeswoman for the European Commission, the bloc's top antitrust regulator, said Thursday that "the clock has been stopped" on the case since March 4. "Information is missing from the file that we require before resuming the investigation," the spokeswoman said.

Orange submitted commitments to the commission on March 6 aimed at addressing its concerns around the deal, according to a statement posted online.

The commission previously halted the merger review for a week in January. Shortly afterward, it decided not to refer the case to Spanish antitrust authorities, saying it was "better placed" to deal with it.

Write to Tom Fairless at tom.fairless@wsj.com and Ruth Bender at Ruth.Bender@wsj.com

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